Thermo Fisher Scientific Beta Actin Recombinant Rabbit Monoclonal Antibody (79E2D7), MonoRab

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

1:5,000-1:10,000

-

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Mammalian cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

79E2D7

Immunogen

A synthetic peptide coupled to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Beta-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to beta-actin provides a useful and specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. GenScript MonoRab™ Beta-Actin Antibody, mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with a synthetic peptide coupled to KLH.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.