#N/A
Recombinant human GDNF protein, expressed in E. coli. High purity (>98%) and low endotoxin (<0.1 ng/µg). Supports dopaminergic neuron survival and neurite outgrowth. Suitable for WB, IHC, ELISA, and functional assays. Lyophilized, carrier-free form for...
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | Assay-dependent | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Functional Assay | Assay-dependent | |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI |
| Molecular Weight | 30.1 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 10 n/mL; determined by the dose-dependent neurite outgrowth of PC-12 cells in the presence of soluble GFR-alpha |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge vial briefly before opening. Reconstitute lyophilized human GDNF in sterile, distilled water to 0.1–0.5 mg/mL. Prepare aliquots and store at ≤ -20°C. Further dilutions should be made in medium or buffered solution containing carrier protein (e.g., PBS with 0.1% BSA).
Storage:
- Lyophilized form: store at 2–8°C, preferably desiccated.
- Reconstituted form: store at ≤ -20°C (avoid frost-free freezer).
- Minimize freeze-thaw cycles.
Target Information
GDNF (Glial cell line-Derived Neurotrophic Factor) is a glycosylated, disulfide-bonded homodimer first identified as a potent survival factor for midbrain dopaminergic neurons. It has been shown to rescue these neurons in animal models of Parkinson’s disease and is approximately 100 times more effective for spinal motor neuron survival than neurotrophins. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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