Thermo Fisher Scientific Glypican 3 Monoclonal Antibody (GT2473)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT2473

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Glypican 3,uniProtId:P51654-1,ncbiNodeId:9606,antigenRange:580,antigenLength:580,antigenImageFileName:MA5-31552_Glypican_3_P51654-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31552_Glypican_3_P51654-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787180

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Chimpanzee (96%), Bovine (81%).

Positive Control: HepG2, HepG2 (24 µg/mL Tunicamycin treatment for 16 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.