Thermo Fisher Scientific Glypican 3 Monoclonal Antibody (GT2473)
인간 Glypican-3 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF에 적합합니다. HepG2 세포를 양성 대조군으로 사용하며, Protein G 정제, PBS/glycerol 저장 buffer 구성. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT2473 |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787180 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Chimpanzee (96%), Bovine (81%).
- Positive Control: HepG2, HepG2 (24 µg/mL Tunicamycin treatment for 16 hr).
- Centrifuge briefly prior to opening the vial.
- Store product as a concentrated solution.
Target Information
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression or modulation of growth in predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in cell division control, growth regulation, and tumor predisposition. Deletion mutations in GPC3 cause Simpson-Golabi-Behmel syndrome (SGBS), characterized by pre- and postnatal overgrowth with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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