Thermo Fisher Scientific ATP6V1A Monoclonal Antibody (GT1561)
ATP6V1A 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 유세포분석에 적합합니다. 인간, 마우스, 랫트에 반응하며 0.8 mg/mL 농도의 액상 형태로 제공됩니다. Protein G 정제, 보존제 무첨가, 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1561 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1A (aa 85–339) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787182 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (90%), Pig (96%), Chicken (82%), Rhesus Monkey (99%), Bovine (98%).
- Positive Control: 293T, HepG2.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase–dependent organelle acidification is necessary for intracellular processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase consists of:
- V1 domain (cytosolic): Three A and three B subunits, two G subunits, plus C, D, E, F, and H subunits. Contains the ATP catalytic site.
- V0 domain (transmembrane): Five different subunits (a, c, c’, c”, and d).
Additional isoforms of many subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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