Thermo Fisher Scientific Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 532, eBioscience
Alexa Fluor 532로 결합된 Rat IgG2b kappa 동형 대조 항체로, 유세포분석(Flow Cytometry)에 적합합니다. 실험 항체의 비특이적 결합 평가용으로 사용되며, 532 nm 레이저로 여기되어 561 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: Assay-Dependent
Control (Ctrl)
- Tested Dilution: Assay-Dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eB149/10H5 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219479 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 660
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
Description:
Rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported:
Rat IgG2b κ Isotype Control Alexa Fluor® 532 has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and human peripheral blood cells.
Use at the same concentration as the experimental antibody.
Fluorochrome Characteristics:
Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm.
Not compatible with the Yellow-Green laser (561 nm).
Recommended filter: 560/14 band pass.
Ensure instrument compatibility before use.
Excitation: 532 nm
Emission: 561 nm
Laser: Green Laser
Target Information
Rat IgG2b encodes the immunoglobulin G2b (IgG2b) subclass of rat antibodies, a major immunoglobulin involved in pathogen neutralization and opsonization.
Commonly used in immunohistochemistry, flow cytometry, and Western blot research applications.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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