
Thermo Fisher Scientific Human IL-7 Recombinant Protein
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인간 IL-7 재조합 단백질로 면역세포 분화 연구에 활용. E. coli 발현 시스템에서 생산된 고순도 단백질(>98%). 세포 증식 활성 검증 완료(ED50 ≤ 0.5 ng/mL). 동결건조 형태로 안정적 보관 가능.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human IL-7 recombinant protein contains 153 amino acids |
| Molecular Weight | 17.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE and HPLC |
| Activity | ED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of mouse 2E8 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
- Use: Human IL-7 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
- Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5–10% FBS or 1–4% high purity BSA (fatty acid free fraction V).
- Stability: Lyophilized product is stable until expiration if stored desiccated below 0ºC. Cytokines diluted as indicated above should be divided into aliquots and stored at -70ºC. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine produced by bone marrow and thymic stromal cells. IL-7 signals through the IL-7 receptor (IL-7R) to promote differentiation of hematopoietic stem cells into lymphoid precursors, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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