Thermo Fisher Scientific Human IL-6 Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent
• Control (Ctrl): Assay-dependent
• Miscellaneous PubMed (Misc): View 2 publications

Product Specifications

Product Specific Information

Mature human IL-6 was expressed in Pichia pastoris X-33 (GenBank Accession # P05231, amino acids 30–212).
The expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VPPGEDSKDV.
Purity is >95% by SDS-PAGE analyses.

Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution:

• Store lyophilized material at 4°C.
• Reconstitute in 0.5 mL of distilled or deionized water.
• Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles.
• PBS with 1% BSA or a neutral medium containing <10% FCS are suitable buffers.

Target Information

IL-6 encodes a cytokine involved in inflammation and B cell maturation.
It acts as an endogenous pyrogen that can induce fever in autoimmune diseases or infections.
The gene is expressed at sites of acute and chronic inflammation, secreted into serum, and induces transcriptional inflammatory responses via the interleukin 6 receptor, alpha.
IL-6 is implicated in inflammation-associated diseases such as diabetes mellitus and systemic juvenile rheumatoid arthritis.
Elevated IL-6 levels are observed in viral infections, including COVID-19.
Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.