Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (1-111A), Biotin, eBioscience

Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (1-111A), Biotin, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

The 1-111A monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. It is expressed on a majority of leukocytes including T, B, NK, and DC cells. B7-H1 acts as a ligand for PD-1, and interaction with PD-1 or PD-L2 (B7-DC) inhibits T and B cell responses.
Preliminary testing suggests that 1-111A and MIH5 (Product # 14-5982) cross-block each other.

Applications Reported:
The 1-111A antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.125 µg per test (defined as antibody amount staining cells in 100 µL final volume). Optimal performance requires titration for assay conditions.
Cell number range: 10⁵–10⁸ cells/test.

Target Information

Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds to PD-1, a receptor known to negatively regulate T-cell receptor (TCR) signaling. PD-L1 engagement decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in many murine and human cancers and can be upregulated by IFN-γ stimulation, suggesting a role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.