Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.25 µg)/test
  • View 7 publications

Product Specifications

Available Formats

• Alexa Fluor 514
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• Alexa Fluor Plus 750
• APC
• eFluor 450
• Request custom conjugation

Product Specific Information

Description:
The MMA monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure to varying degrees, while lymphocytes are negative.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  Storage: up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Optical Properties:

• Excitation: 488 nm
• Emission: 710 nm
• Laser: Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1)
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) expression show calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma autoaggregation. Sialyl-Le(x) and sialyl-Le(a) are selectin ligands. CD15 expression is widely used in the diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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