Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 615 / 737 / 805
• Brilliant Violet 711 / 786
• eFluor 450
• FITC
• NovaFluor Blue 510 / 610-30S
• NovaFluor Red 685 / 710
• NovaFluor Yellow 570 / 590 / 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. CD24 is variably glycosylated, resulting in heterogeneity of molecular mass across different lineages. Expression of CD24 is used to distinguish stages of B lymphopoiesis in mouse bone marrow. CD24 has been reported to bind P-selectin (CD62P).

Applications Reported: Flow cytometric analysis and in vitro functional assays.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes and thymocytes. Recommended ≤0.125 µg/test (100 µL final volume). Optimal titration advised.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells to mature B cells. Plasma cell expression is low or absent. CD24 is also expressed on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may play roles in B-cell proliferation, maturation, and autoimmunity regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.