Thermo Fisher Scientific F(ab`)2-Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
TRITC 결합 Donkey anti-Rat IgG (H+L) F(ab`)2 형태의 고도로 교차흡착된 2차 항체로, Rat IgG 검출에 적합. ICC/IF에 1 µg/mL로 사용 가능하며, 높은 특이성과 낮은 교차반응 제공. 552/578 nm 형광 특성으로 민감한 검출에 이상적.
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Thermo Fisher Scientific F(ab`)2-Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoid freeze/thaw cycles, store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536016 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, and sheep IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Product has been overfilled to ensure complete recovery.
- Stable for 1 year from date of receipt.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins.
They are useful in detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and are further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- HRP
- Custom conjugation available upon request
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