Thermo Fisher Scientific F(ab`)2-Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC
FITC로 표지된 Donkey anti-Rat IgG (H+L) 2차 항체로, F(ab`)2 fragment 형태입니다. 높은 교차흡착 처리로 비특이적 결합을 최소화하며, ICC/IF 등 형광 이미징에 최적화되어 있습니다. 498/517 nm의 여기/방출 특성을 가집니다.
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Applications
Tested Dilution
- Immunocytochemistry (ICC/IF): 2.5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | FITC (Fluorescein) |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | 4°C (short term), -20°C (long term), avoid freeze/thaw, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536012 |
| Target | IgG |
| Cross Adsorption | Bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG |
| Antibody Form | F(ab`)2 Fragment |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance assay versatility by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and provide greater sensitivity through signal amplification.
Typically, these antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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