
Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC
쥐 IgG(H+L)에 특이적인 염소 유래 F(ab')2 단편 2차 항체로, FITC 형광 표지되어 있습니다. 세포면역형광(ICC/IF) 및 유세포분석(Flow Cytometry)에 적합하며, 사람 및 마우스 IgG에 대해 교차흡수 처리되어 비특이적 결합을 최소화합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 2.5 µg/mL |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536018 |
| Target | IgG |
| Cross Adsorption | Against human and mouse IgG |
| Antibody Form | F(ab`)2 Fragment |
Available Conjugate Formats
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- APC
- Biotin
- Cyanine3
- Cyanine5
- DSB-X Biotin
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- HRP
- PE
- Texas Red-X
- TRITC
- Request custom conjugation available
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful in detection, sorting, or purification of rat immunoglobulins. Secondary antibodies provide increased versatility by enabling various detection systems (e.g., HRP, AP, fluorescence) and can improve sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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