
Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab`)2 형태의 Goat anti-Rat IgG (H+L) 교차흡착 2차 항체로, Rat IgG 검출에 높은 특이성과 민감도를 제공. WB, IHC, ELISA에 사용 가능하며, 인간 및 마우스 IgG에 대한 교차 반응 최소화. 동결건조 형태로 안정적 보관 가능.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2536017 |
| Target | IgG |
| Cross Adsorption | Against human and mouse IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8 °C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Based on Immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse. Highly cross-adsorbed against mouse IgG.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins.
Useful for detection, sorting, or purification of rat immunoglobulins.
Secondary antibodies offer versatility for multiple detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification.
Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, APC, Biotin, Cyanine3, Cyanine5, DSB-X Biotin
- DyLight 488, 550, 594, 650, 680, 755, 800
- FITC, PE, Texas Red-X, TRITC
- Custom conjugation available upon request
Usage Note
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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