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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

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HRP 결합 F(ab`)2 형태의 Goat anti-Rat IgG (H+L) 교차흡착 2차 항체로, Rat IgG 검출에 높은 특이성과 민감도를 제공. WB, IHC, ELISA에 사용 가능하며, 인간 및 마우스 IgG에 대한 교차 반응 최소화. 동결건조 형태로 안정적 보관 가능.

카탈로그번호
A24549
판매단위
pk
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마지막 업데이트 2025. 08. 04. 오후 10:08
Thermo Fisher Scientific A24549 F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP 500 ug pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Applications and Tested Dilution

Application Tested Dilution
Western Blot (WB) 1:1,000–1:20,000
Immunohistochemistry (IHC) 1:500–1:20,000
ELISA 1:500–1:20,000

Product Specifications

항목 내용
Species Reactivity Rat
Host / Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Gamma Immunoglobins Heavy and Light chains
Conjugate HRP
Form Lyophilized
Concentration 2.5 mg/mL
Purification Affinity Chromatography
Storage Buffer PBS, pH 7.2, with 1% BSA
Contains 0.1% ProClin 150
Storage Conditions Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles.
Shipping Conditions Wet ice
RRID AB_2536017
Target IgG
Cross Adsorption Against human and mouse IgG
Antibody Form F(ab`)2 Fragment

Product Specific Information

Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8 °C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Based on Immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse. Highly cross-adsorbed against mouse IgG.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins.
Useful for detection, sorting, or purification of rat immunoglobulins.
Secondary antibodies offer versatility for multiple detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity through signal amplification.
Typically generated by immunizing the host animal with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.

Additional Formats Available

  • Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
  • AP, APC, Biotin, Cyanine3, Cyanine5, DSB-X Biotin
  • DyLight 488, 550, 594, 650, 680, 755, 800
  • FITC, PE, Texas Red-X, TRITC
  • Custom conjugation available upon request

Usage Note

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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