Thermo Fisher Scientific ULK2 Polyclonal Antibody
ULK2 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. 인간 및 생쥐에서 반응하며, 자가포식 관련 연구에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 693–968 of ULK2 (Uniprot ID: Q8IYT8) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.24 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_11153516 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1, NIH-3T3
- Predicted reactivity: Mouse (94%), Rat (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ULK2 (also known as ATG1B) is a serine/threonine protein kinase acting at the upstream step of autophagosome formation. Knockout of ULK2 causes severe defects in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shares high homology with ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling, essential for autophagy. ULK2 is also implicated in early neuronal growth and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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