Thermo Fisher Scientific Phospho-CDC25b (Ser42) Polyclonal Antibody
인산화된 CDC25B(Ser42)를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(Paraffin) 검증 완료. 항원 친화 크로마토그래피 정제, 액상 형태. 세포주기 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide surrounding -LGSPV- of human CDC25B protein containing a phosphorylation site at Serine 42 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinoma
- Cellular location: Cytoplasmic
Target Information
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression.
Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in an ECT2-dependent manner.
Directly dephosphorylates CDK1 and stimulates its kinase activity.
The three isoforms seem to have different levels of activity.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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