Thermo Fisher Scientific CDC25b Polyclonal Antibody
Rabbit polyclonal antibody recognizing human CDC25B protein. Validated for WB and IHC(P) applications. Supplied as an unconjugated liquid at 0.5 mg/mL. Suitable for research use in cell cycle and mitotic regulation studies.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide surrounding -LGSPV- of human CDC25B protein without the phosphorylation site at Serine 42 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinoma
- Cellular location: Cytoplasmic
Target Information
Tyrosine protein phosphatase functioning as a dosage-dependent inducer of mitotic progression. Required for G2/M phase progression and cytokinesis in an ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms exhibit different activity levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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