Thermo Fisher Scientific eIF3i Polyclonal Antibody
Thermo Fisher Scientific의 eIF3i Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 항체입니다. Western blot 및 IHC(P) 실험에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 단백질 합성 개시 인자 eIF3 복합체 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human eIF3i. Recombinant protein control fragment (Product #RP-94035) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640967 |
Product Specific Information
Immunogen sequence:
KYNREGDLLF TVAKDPIVNV WYSVNGERLG TYMGHTGAVW CVDADWDTKH VLTGSADNSC RLWDCETGKQ LALLKTNSAV RTCG
- Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 87%
Target Information
The eIF3i protein is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, essential for multiple steps in the initiation of protein synthesis.
This complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It also promotes mRNA recruitment and scanning for AUG recognition, and is involved in the disassembly and recycling of post-termination ribosomal complexes, preventing premature joining of ribosomal subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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