Thermo Fisher Scientific Cytokeratin 19 (KRT19) Recombinant Rabbit Monoclonal Antibody (KRT19, 1959R)
Recombinant rabbit monoclonal antibody targeting Cytokeratin 19 (KRT19). Validated for WB and IHC applications. High specificity for human and mouse samples. Supplied as liquid, unconjugated form at 1 mg/mL concentration. Suitable for epithelial tissue...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Parameter | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | KRT19, 1959R |
| Immunogen | Recombinant human full-length KRT19 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Cytokeratin 19 is part of a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight from 40–68 kDa and isoelectric pH between 4.9–7.8.
The expression patterns of cytokeratin subtypes are used to distinguish different types of epithelial malignancies.
Cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 3880-RBM9-P1ABX_Cytokeratin_19_P08727-1_Rabbit.svg, 3880-RBM9-P1ABX_Cytokeratin_19_P08727-1_Rabbit_PDP.jpeg)
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