Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), FITC, eBioscience
CD29 (Integrin beta 1) 단백질을 인식하는 FITC 결합 아르메니아 햄스터 단클론 항체. 마우스 및 랫트 시료에 반응하며, Flow Cytometry에 최적화됨. 세포 부착, 이동, 분화 관련 연구에 적합. 488 nm/520 nm 파장에서 검출 가능.
- 카탈로그번호
- 11-0291-8x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 72 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioHMb1-1 (HMb1-1) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572448 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 755
- NovaFluor Yellow 660
- NovaFluor Yellow 700
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 binds non-covalently with alpha integrins (CD49a-f) to form VLA-1 through VLA-6 complexes, as well as with CD51. These integrin heterodimers mediate cellular adhesion, trafficking, proliferation, and differentiation.
Applications Reported:
- Flow cytometric analysis
Applications Tested:
- Tested by flow cytometric analysis of mouse bone marrow cells
- ≤ 1 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Recommended to titrate for optimal performance
Fluorescence Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
ITGB1 (Integrin Subunit Beta 1, CD29) is a 110 kDa cell surface glycoprotein expressed by various cell types including leukocytes. It forms non-covalent heterodimers with multiple alpha chains (α1–α6), determining the binding properties of beta1 (VLA) integrins. ITGB1 plays a key role in cell adhesion, migration, and immune cell trafficking. It is involved in tissue damage and inflammatory responses, and its expression correlates with multidrug resistance in tumor cells. Two major isoforms exist: beta1A (ubiquitous) and beta1D (cardiac/skeletal muscle).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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