Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), FITC, eBioscience
마우스 CD31(PECAM-1)을 인식하는 단클론 항체로, FITC로 표지되어 있습니다. 유세포분석 및 동결 조직 면역조직화학에 적합하며, 488 nm 레이저에서 여기됩니다. 내피세포 및 백혈구 표면 단백질 연구에 활용됩니다.
- 카탈로그번호
- 11-0311-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 38 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | 20 µg/mL | 6 |
| Immunocytochemistry (ICC/IF) | - | 14 |
| Flow Cytometry (Flow) | 1 µg/test | 135 |
| ChIP assay (ChIP) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse, Zebrafish |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465011 |
Additional Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available upon request
Product Specific Information
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on all leukocytes and platelets. CD31 plays a critical role in adhesion, cell-cell and cell-matrix interactions, and signal transduction. CD38 has been reported to bind to CD31, and homotypic CD31 interactions are important for cellular adhesion and signaling.
Applications Reported:
Flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested:
- Flow cytometry: ≤1 µg/test (defined as the amount of antibody staining a cell sample in 100 µL; 10⁵–10⁸ cells/test).
- Immunohistochemistry (Frozen): ≤20 µg/mL.
Titrate antibody for optimal performance.
Fluorescence Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD31 (Platelet Endothelial Cell Adhesion Molecule-1, PECAM-1) functions as an inhibitory coreceptor regulating T and B cell signaling via dual ITIM motifs. It is widely expressed in the vascular compartment, primarily at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 is involved in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immunoregulation, autoimmunity, and thrombosis, serving as a key regulator in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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