Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Recombinant Rabbit Monoclonal Antibody (BTN, 2032R)
Thermo Fisher Scientific의 Biotin (Vitamin B7) recombinant rabbit monoclonal antibody (BTN, 2032R). IHC 및 ISH에 적합하며 높은 특이성과 재현성을 제공. HEK293 발현 시스템 사용, Protein A로 정제된 액상 형태. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
In Situ Hybridization (ISH)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BTN, 2032R |
| Immunogen | Biotinylated sheep immunoglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7.
It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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