Thermo Fisher Scientific Thyroglobulin Monoclonal Antibody (2H11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:200

-

Immunohistochemistry (IHC)

1:50-1:200

-

ELISA (ELISA)

1:20,000-1:100,000

-

Immunoprecipitation (IP)

1:50-1:200

-

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2H11

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.6 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.5M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.

Target Information

The human thyroglobulin (hTg) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti-thyroglobulin autoantibodies were the first factor to be discovered. Anti-hTG is found in all thyroid autoimmune diseases (Hashimoto thyroiditis, Graves diseases), with the highest level observed in Hashimoto thyroiditis. Anti-hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.