Thermo Fisher Scientific Thyroglobulin Monoclonal Antibody (2H11)
인간 Thyroglobulin을 인식하는 단일클론 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. Mouse IgG1 형식이며 Protein A로 정제됨. 인간, 개, 생쥐, 랫트 반응성. 연구용으로만 사용.
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Thermo Fisher Scientific Thyroglobulin Monoclonal Antibody (2H11)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:200 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H11 |
| Immunogen | Protein A purified monoclonal antibody produced by repeated immunizations with human thyroglobulin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.6 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.5M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross-reactivity with thyroglobulin from mouse, rat, and dog will occur. Cross-reactivity with thyroglobulin from other sources has not been determined.
Target Information
Human thyroglobulin (hTg) is a high molecular weight glycoprotein (605 kDa) found in thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones T4 and T3. Thyroid disorders often have an autoimmune origin, and anti-thyroglobulin autoantibodies were the first such factor discovered. Anti-hTG is found in all thyroid autoimmune diseases (Hashimoto thyroiditis, Graves disease), with the highest levels observed in Hashimoto thyroiditis. Anti-hTG is also characteristic of thyroid cancer and can be used for monitoring cancer patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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