Thermo Fisher Scientific Keratin Monoclonal Antibody (C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:200

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C11

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 6,uniProtId:P02538-1,ncbiNodeId:9606,antigenRange:1-564,antigenLength:564,antigenImageFileName:200-301-390_Cytokeratin_6_P02538-1_House_mouse.svg,antigenImageFileNamePDP:200-301-390_Cytokeratin_6_P02538-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Cytokeratin 6,uniProtId:P48668-1,ncbiNodeId:9606,antigenRange:1-564,antigenLength:564,antigenImageFileName:200-301-390_Cytokeratin_6_P48668-1_House_mouse.svg,antigenImageFileNamePDP:200-301-390_Cytokeratin_6_P48668-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:Cytokeratin 6,uniProtId:P04259-1,ncbiNodeId:9606,antigenRange:1-564,antigenLength:564,antigenImageFileName:200-301-390_Cytokeratin_6_P04259-1_House_mouse.svg,antigenImageFileNamePDP:200-301-390_Cytokeratin_6_P04259-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts specifically with keratins from human tissues and derived cell lines. This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans. Cross reactivity with keratins from other sources has not been determined. No reaction is expected against other filament proteins including vimentin, desmin and neurofilament protein.

Target Information

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.