Thermo Fisher Scientific Keratin Monoclonal Antibody (C11)
인간 각질 단백질(Keratin)을 특이적으로 인식하는 마우스 단클론 항체(C11). Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. Protein A 정제, 1.3 mg/mL 농도, -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C11 |
| Immunogen | Protein A purified monoclonal antibody produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.3 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts specifically with keratins from human tissues and derived cell lines, including keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa), and keratin 19 (40 kDa).
Cross-reactivity with keratins from other sources has not been determined.
No reaction is expected against other filament proteins such as vimentin, desmin, and neurofilament protein.
Target Information
Keratins 6 and 16 are expressed in keratinocytes undergoing rapid turnover in the suprabasal region (hyperproliferation-related keratins).
Keratin 6 is found in hair follicles and suprabasal cells of internal stratified epithelia and epidermis, both in normal and hyperproliferative situations.
Epidermal injury induces keratinocytes expressing CK6 and CK16.
CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas and is associated with differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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