Thermo Fisher Scientific Insulin Monoclonal Antibody (2D11.H5)
Western blot, IHC, ELISA 등 다양한 응용에 적합한 인간 인슐린 특이 단클론 항체. 인간 및 돼지 인슐린에 반응하며, 마우스·랫트 인슐린에는 비반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:200 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:50–1:200 |
| Dot Blot (DB) | 1:400 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D11.H5 |
| Immunogen | Protein A purified monoclonal antibody produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity:
Reacts specifically with insulin from human and swine sources.
No cross-reactivity with insulin from mouse or rat.
Cross-reactivity with insulin from other sources not determined.
Target Information
Insulin is a peptide hormone secreted by pancreatic β-cells that regulates carbohydrate, protein, and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It promotes glycogen storage, triglyceride formation, and protein/nucleic acid synthesis.
Insulin is a 51-amino acid polypeptide derived from proinsulin, which is cleaved into A and B chains linked by disulfide bonds and one molecule of C-peptide.
Insulin deficiency leads to diabetes mellitus, a major cause of morbidity and mortality. Insulin is also found in β-cell tumors such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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