Thermo Fisher Scientific Phospho-SMC1 (Ser957) Monoclonal Antibody (5D11.G5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

-

Immunohistochemistry (IHC)

2.5 µg/mL

-

ELISA (ELISA)

1:20,000-1:100,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5D11.G5

Immunogen

This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SMC1,uniProtId:Q14683-1,ncbiNodeId:9606,antigenRange:951-962,antigenLength:1233,antigenImageFileName:200-301-397_SMC1_Q14683-1_House_mouse.svg,antigenImageFileNamePDP:200-301-397_SMC1_Q14683-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against human SMC1 and is useful in determining its presence in various assays. This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. Minimal reactivity is observed against the non-phosphorylated epitope. Reactivity is observed against human and mouse SMC1. Cross reactivity with SMC1 from other eukaryotic sources has not been tested.

Target Information

Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromo proteins are conserved in eukaryotes and lead to mitotic chromosome segregatior defects. This suggests a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. Mutations in the gene can lead to Cornelia de Lange syndrome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.