Thermo Fisher Scientific Phospho-SMC1 (Ser957) Monoclonal Antibody (5D11.G5)
SMC1 단백질의 Ser957 인산화 형태를 특이적으로 인식하는 마우스 단일클론 항체. Western blot, IHC, ELISA 등 다양한 응용에 적합. Human 및 Mouse 반응성. Protein G 정제, 1 mg/mL 농도, 액상 형태로 제공.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:2,000 | - |
| Immunohistochemistry (IHC) | 2.5 µg/mL | - |
| ELISA | 1:20,000–1:100,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D11.G5 |
| Immunogen | Synthetic peptide corresponding to aa 951–962 of human SMC1 (UniProt ID: Q14683-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This monoclonal antibody is directed against human SMC1 and recognizes the phosphorylated epitope in native and over-expressed proteins from various tissues and extracts.
Minimal reactivity is observed against the non-phosphorylated epitope.
Reactivity is observed against human and mouse SMC1.
Cross-reactivity with SMC1 from other eukaryotic sources has not been tested.
Target Information
Structural Maintenance of Chromosomes (SMC) family proteins are essential for nuclear processes requiring chromosome structural changes, including mitotic organization, DNA recombination and repair, and transcriptional repression.
SMC1 and SMC3 form a heterodimeric complex crucial for sister chromatid cohesion during metaphase.
Mutations in SMC1 can cause Cornelia de Lange syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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