Thermo Fisher Scientific SCD Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/ml

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region of human SCD.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2183105

Product Specific Information

Recommended positive controls: human liver, Jurkat.

Predicted reactivity: Cow: 92%; Dog: 85%; Goat: 85%; Guinea Pig: 85%; Mouse: 77%; Rat: 77%; Sheep: 85%.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5., have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.