Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), PE-Cyanine7, eBioscience
마우스 NK1.1 항원을 인식하는 PK136 단클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. NK 세포 및 일부 T 세포 분석에 적합하며, 유세포분석용으로 검증되었습니다. 4°C 암소 보관, 동결 금지. 연구용 전용 제품입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 105 publications |
| Affinity Purification (AP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469664 |
Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer (NK) cells and a subset of T cells in NK1.1-positive mouse strains (e.g., C57BL and NZB). Common strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For these strains, use the DX5 monoclonal antibody (Product #14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by most cells, with minor DX5⁺PK136⁻ and DX5⁻PK136⁺ subpopulations.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test.
A test is defined as the antibody amount staining a cell sample in 100 µL volume. Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Light Sensitivity: PE-Cyanine7 is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal signal loss.
Excitation: 488–561 nm
Emission: 775 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161) is a C-type lectin-like receptor expressed on natural killer (NK) cells and some T lymphocytes. It plays a role in NK cell cytotoxicity and cytokine secretion. The KLRB1 protein contains extracellular C-type lectin motifs, a transmembrane domain, and a cytoplasmic domain. It is a type II membrane protein with an external C-terminus. NKR-P1A recognizes LLT1 (Lectin Like Transcript-1) as its ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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