Thermo Fisher Scientific BUB1B Monoclonal Antibody (OTI5D9), TrueMAB
BUB1B 단백질을 인식하는 Mouse Monoclonal Antibody로 Western blot, IHC, ICC/IF에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 세포분열 체크포인트 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D9 |
| Immunogen | Full-length protein expressed in 293T cell transfected with human BUB1B expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
BUBR1 is a member of the Ser/Thr protein kinase family and a key component of the mitotic checkpoint, which delays anaphase until all chromosomes are properly attached to the mitotic spindle.
Defective spindle checkpoints contribute to genetic instability and malignant transformation.
Human cells express two related checkpoint kinases derived from the BUB1 gene: hBUBR1 and hBUB1. Both monitor kinetochore-microtubule interactions during mitosis.
hBUBR1 prevents premature anaphase entry and is essential for normal mitotic progression.
BUBR1 has been detected in human cancers and identified as a binding partner of BCSG1, which may accelerate breast cancer progression by impairing checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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