Thermo Fisher Scientific CHOP Polyclonal Antibody
CHOP 단백질(DITT3)을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성 보유. 고순도 Affinity chromatography 정제, 1 mg/mL 농도. PBS/glycerol buffer에 보관하며 -20°C에서 안정적 저장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | — |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | — |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DDIT3 (Accession P35638), corresponding to amino acid residues L6–D56 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851714 |
Product Specific Information
Antibody detects endogenous levels of total DDIT3.
Target Information
GADD153 is a small nuclear protein capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Conversely, the C/EBP–GADD153 dimer gains binding activity to other non-classical C/EBP stress-related targets. Under normal cellular conditions, this protein is not expressed at detectable levels but is highly upregulated during cellular or ER stress. Examples of GADD153-inducing stress include treatment with tunicamycin, nutrient starvation, and reducing agents that interfere with calcium flux across the ER membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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