Thermo Fisher Scientific Cytochrome C Polyclonal Antibody
사이토크롬 C 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 미토콘드리아 내막 간격 내 단백질 검출에 사용되며, 세포 사멸 연구에 유용합니다. 항원 친화 크로마토그래피로 정제된 액상 형태입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide of human Cytochrome C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human cervical carcinoma tissue
- Cellular location: Mitochondrion matrix
Target Information
Cytochrome C is an electron-transporting protein located within the intermembrane space of mitochondria, where it plays a critical role in oxidative phosphorylation and cellular ATP production.
Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, cytochrome C binds apoptotic protease activating factor, which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, a key effector of apoptosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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