Thermo Fisher Scientific Phospho-ELK-1 (Ser383) Polyclonal Antibody
ELK1 단백질의 Ser383 인산화 상태를 검출하는 폴리클로날 항체입니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 사용 가능하며, 인간·마우스·랫트 반응성을 가집니다. 항원 친화 크로마토그래피로 정제된 액상 형태로 제공됩니다.
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Thermo Fisher Scientific Phospho-ELK-1 (Ser383) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser282 of human ELK-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human breast carcinoma
- Cellular location: Nuclear
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, and the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity. Interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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