Thermo Fisher Scientific Integrin beta 7 Monoclonal Antibody (FIB504), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 1 publication

Product Specifications

Additional Formats

• Unconjugated
• FITC
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

Description:
The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7, a 130 kD glycoprotein that associates with integrin alpha 4 (CD49d) to form the alpha4beta7 integrin (LPAM-1), expressed on intraepithelial lymphocytes. It also associates with alphaE (CD103) to form the alphaEbeta7 integrin (HML-1), expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes.
Main ligands for integrin alpha4beta7 include VCAM-1 (CD106), MAdCAM-1, and fibronectin, while the ligand of integrin alphaEbeta7 is E-cadherin (CD324).
Integrin beta 7 plays an important role in leukocyte adhesion to endothelial cells and transmigration during inflammatory responses. The FIB504 antibody has been reported to exhibit blocking activity.

Applications Reported:
This antibody is suitable for flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Can be used at ≤0.125 µg per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form LPAM-1, expressed on intraepithelial lymphocytes, and with alpha E (CD103) to form HML-1, expressed on T cells near mucosal epithelium.
Ligands include VCAM-1 (CD106), MAdCAM-1, fibronectin, and E-cadherin (CD324).
Integrin beta 7 is essential for leukocyte adhesion and transmigration during inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.