Thermo Fisher Scientific XAF1 Polyclonal Antibody
XAF1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot과 Immunocytochemistry에 적합하며, 다양한 XAF1 isoform을 검출. 항원 친화 크로마토그래피로 정제된 액상 항체로, 인체 유래 시료 분석에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1.0 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage, store at –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152746 |
Product Specific Information
- This antibody recognizes multiple isoforms of XAF-1.
- In Western blot applications, a doublet is detected at approximately 30 kDa.
- A suggested positive control is human spleen tissue lysate.
- PA5-20090 can be used with blocking peptide PEP-0208.
Target Information
The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by inhibiting distinct caspases.
The X-linked Inhibitor of Apoptosis (XIAP) is a key member of this family.
XIAP’s caspase-inhibiting activity is negatively regulated by XIAP-associated factor 1 (XAF1) and Smac/DIABLO.
XAF1 binds to XIAP and antagonizes its inhibitory activity on caspases, leading to redistribution of XIAP from the cytosol to the nucleus.
XAF1 is ubiquitously expressed in normal tissues but is often reduced or absent in various cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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