Thermo Fisher Scientific Bim Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to amino acids near the center of human Bim

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152570

Product Specific Information

A suggested positive control is K562 cell lysate.

PA5-20089 can be used with blocking peptide PEP-0207.

The PA5-20089 immunogen is located within amino acids 80-130 of BIM.

Predicted molecular ~ 22kD. In Western blot applications, this antibody has been observed to detect a band at: 22kD.

Predicted species reactivity based on immunogen sequence: Rat: (100%).

Target Information

BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.