Thermo Fisher Scientific MIA Polyclonal Antibody, Biotin, PeproTech

Applications

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MIA. Anti-Human MIA-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hMIA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIA (500-P243) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hMIA.

Western Blot:
To detect hMIA by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hMIA is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P243BT-1MG will be provided as 2 × 500 µg.

Target Information

Tumorigenesis is mediated by a network of membrane, cytosolic, and nuclear-associated factors regulating proliferation and cell-matrix interaction through signaling cascades.
Melanoma inhibitory activity (MIA, also known as cartilage-derived retinoic acid-sensitive protein, CD-RAP) is a Src homology 3 (SH3)-like domain-containing protein secreted from chondrocytes and malignant melanoma cells.
MIA is translated as a 131-amino acid pro-form and processed into a mature 107-amino acid protein after cleavage of a secretion signal. It is expressed during chondrogenesis and in mature chondrocytes, suggesting a role in maintaining normal cartilage cell phenotype.
MIA mRNA is present in carcinomas of the colon, ovary, kidney, and head/neck, and may serve as a marker for monitoring melanomic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.