Thermo Fisher Scientific Artemin Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Artemin. Anti-Human Artemin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hArtemin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Artemin (500-P245) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hArtemin.

Western Blot:
To detect hArtemin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hArtemin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P245BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a member of the glial cell line-derived neurotrophic factor (GDNF) family of ligands within the TGF-beta superfamily. GDNFs have neurotrophic properties and potential applications in gene therapy for neurodegenerative diseases. Artemin supports survival of peripheral and dopaminergic CNS neurons, acting as a ligand for the RET receptor and using GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.