Takara T7 RNA Polymerase, HQ

T7 RNA Polymerase, HQ is a high-quality DNA-dependent RNA polymerase that exhibits a high processivity and transcription frequency, comparable to RUO-grade T7 RNA Polymerase ver.2.0 (Cat. #2541A). However, T7 RNA Polymerase, HQ has stricter quality standards than the RUO grade (see the table below) with a high volume (1 mL/tube) for your scale-up process.

A GMP grade T7 RNA Polymerase is available upon request. Please contact us (Inside_Sales@takarabio.com) for more information.

Do you want to explore OEM options? Contact OEM Services & Support (oem@takarabio.com).

T7 RNA Polymerase, HQ is a high-quality DNA-dependent RNA polymerase that exhibits a high processivity and transcription frequency, comparable to RUO-grade T7 RNA Polymerase ver.2.0 (Cat. #2541A). However, T7 RNA Polymerase, HQ has stricter quality standards than the RUO grade (see the table below) with a high volume (1 mL/tube) for your scale-up process.

T7 RNA Polymerase, HQ exhibits high specificity for the bacteriophage T7 promoter sequence 5′-TAATACGACTCACTATA-3′. The enzyme uses double-stranded DNA containing T7 promoter sequence as a template and incorporates labeled or unlabeled nucleoside triphosphates to synthesize a single-stranded RNA transcript.

This enzyme is intended for use in the preparation of active pharmaceutical ingredients (APIs) for non-clinical trials, as well as for the process development of drug manufacturing under good manufacturing practice (GMP) regulations. Its similarity to GMP grade enzymes supports a smooth transition from RUO to GMP grade RNA manufacturing.

It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation. This makes it suitable for use in applications where there is a risk of immunogenic reactions.

A GMP grade T7 RNA Polymerase is available upon request. Please contact us (Inside_Sales@takarabio.com) for more information.

Do you want to explore OEM options? Contact OEM Services & Support (oem@takarabio.com).

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Overview

This kit can be used for synthesis of RNA to be used in many applications:

1. RNA for enzymatic capping
2. Capped mRNA using a cap analog
3. Long non-coding RNA
4. Guide RNA
5. siRNA precursors
6. RNA standard templates for RT-qPCR
7. Highly labeled RNA probes

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Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.