Thermo Fisher Scientific MCM10 Polyclonal Antibody
MCM10 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 적합. Human, Mouse, Rat에 반응하며, 핵 내 단백질 검출에 사용 가능. 고순도 Affinity Chromatography 정제, -20°C 보관, 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 616–875 of human MCM10 (NP_877428.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.54 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914646 |
Product Specific Information
- Positive test control: Jurkat
- Subcellular location: Nucleus
Immunogen sequence:
RLEGAPATMT PKLGRGVLEG DDVLFYDESP PPRPKLSALA EAKKLAAITK LRAKGQVLTK TNPNSIKKKQ KDPQDILEVK ERVEKNTMFS SQAEDELEPA RKKRREQLAY LESEEFQKIL KAKSKHTGIL KEAEAEMQER YFEPLVKKEQ MEEKMRNIRE VKCRVVTCKT CAYTHFKLLE TCVSEQHEYH WHDGVKRFFK CPCGNRSISL DRLPNKHCSN CGLYKWERDG MLKEKTGPKI GGETLLPRGE EHAKFLNSLK
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) involved in the initiation of eukaryotic genome replication.
The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in replication fork formation and recruitment of DNA replication-related proteins.
It interacts with MCM2, MCM6, and ORC2, and is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner.
Studies in Xenopus suggest chromatin binding occurs after pre-RC assembly and before origin unwinding.
Alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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