Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), eFluor 450, eBioscience
마우스 CD25(IL-2Rα)에 특이적인 단클론 항체로, eFluor 450 형광체로 결합되어 Flow Cytometry에 최적화됨. IL-2 결합 억제 및 림프구 활성화 연구에 활용 가능. 405 nm 레이저로 여기, 445 nm에서 방출. 연구용으로만 사용.
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Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 90 publications |
| Neutralization (Neu) | - | View 2 publications |
| Functional Assay (Functional) | - | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10698312 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 506, FITC
- Functional Grade
- NovaFluor Blue 585, 610-70S, 660-120S, 760
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα).
CD25 is expressed by early progenitors of T and B lineage cells and by activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 with low affinity, but associates with CD122 (IL-2Rβ) and CD132 (common γ chain) to form the high-affinity IL-2 receptor.
Binding of IL-2 to both high and low affinity IL-2 receptor classes is inhibited by the PC61.5 antibody.
CD25 plays a role in lymphocyte differentiation, activation, and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Optimize antibody titration for best performance.
Fluorophore Information:
eFluor™ 450 is an alternative to Pacific Blue™.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
Ensure instrument compatibility.
Filtered post-manufacturing (0.2 µm).
Target Information
CD25 (IL2RA) encodes the interleukin-2 receptor alpha chain, part of a heterotrimeric complex with IL2RB and IL2RG.
This receptor mediates IL-2 signaling involved in T and B lymphocyte proliferation.
IL-2 functions as a growth factor, differentiation factor, and regulator of apoptosis.
IL-2 also stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation.
Mutations in IL2RA can lead to immune dysregulation and colitis-like disease in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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