Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), PE, eBioscience
마우스 CD54(ICAM-1)을 인식하는 eBioKAT-1 단클론 항체로, PE 형광표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 염증 반응 연구에 활용됩니다. 0.125 µg/test로 사용 가능하며, 4°C 보관 및 동결 금지.
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Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), PE, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- Publications: View 6 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioKAT-1 (KAT-1, KAT1) |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_529540 |
Available Formats:
- Unconjugated
- APC
- Functional Grade
- Super Bright 436
- Custom conjugation available
Product Specific Information
The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, which is essential for transendothelial migration of T cells. ICAM-1-deficient mice show reduced leukocyte migration and impaired inflammatory responses.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.125 µg/test
- Recommended Use: Titrate for optimal performance
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. It has seven potential N-linked glycosylation sites and shares homology with ICAM-2 and ICAM-3. ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.
ICAM-1 is expressed by activated endothelial cells and various immune cells (B, T, monocytes, macrophages, dendritic cells, neutrophils), with levels increasing upon inflammation. It is also found in carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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