Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available

Product Specific Information

Description:
The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (Integrin α6), also known as very late activation antigen 6 (VLA-6 α chain) and platelet gpIc. CD49f is a 120 kDa transmembrane protein that associates with CD29 (Integrin β1) to form the VLA-6 complex or with CD104 (Integrin β4) to form the α6β4 complex. It is expressed primarily on T cells, monocytes, platelets, epithelial, and endothelial cells, and has also been found on germinal center B cells.
This antibody cross-reacts with human, mouse, and bovine integrin α6 and can block binding of integrin α6 to laminin.

Applications Reported:

• Flow cytometric analysis

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.125 µg/test (100 µL final volume). Optimal titration is advised per assay.
Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD49f (Integrin α6, ITGA6) encodes the integrin α6 chain, forming heterodimers with β1 or β4 subunits (e.g., VLA-6 or TSP180). Integrins mediate cell adhesion and signaling between the extracellular matrix (ECM) and cytoskeleton, influencing cell morphology, mobility, and proliferation.
CD49f is predominantly expressed in epithelial tissues and plays a key role in hemidesmosome structure. Over 18 α and 8 β subunits have been identified in mammals, enabling diverse integrin combinations and functions, including cell aggregation via ICAM interactions.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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