Thermo Fisher Scientific DTYMK Monoclonal Antibody (OTI2B1), TrueMAB
DTYMK 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합. HEK293T 세포에서 생산된 full-length human recombinant protein을 면역원으로 사용. 동결건조 형태, 1 mg/mL 농도, 연구용으로만 사용 가능.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B1 |
| Immunogen | Full length human recombinant protein of human DTYMK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, it is recommended to add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.
DTYMK is essential for DNA synthesis and is an important intermediate enzyme in the pathway of many pyrimidine analog drugs, including 5-Fluorouracil (5-FU), a chemotherapeutic drug used for several solid tumors.
The human DTYMK gene was isolated via functional complementation of a Saccharomyces cerevisiae cell cycle mutant, cdc8.
DTYMK mRNA levels and enzyme activities are regulated by cell cycle and cell growth pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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