Thermo Fisher Scientific DTYMK Monoclonal Antibody (OTI1G8), TrueMAB
DTYMK 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western Blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 인간 시료에 반응하며, Lyophilized 형태로 제공. DNA 합성 관련 효소 연구 및 항체 결합 실험에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G8 |
| Immunogen | Full length human recombinant protein of human DTYMK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.
DTYMK is essential for DNA synthesis and serves as an intermediate enzyme in the pathway of several pyrimidine analog drugs, including 5-Fluorouracil (5-FU), used in chemotherapy for solid tumors.
The human DTYMK gene was identified via functional complementation of a Saccharomyces cerevisiae cell cycle mutant (cdc8).
DTYMK mRNA levels and enzyme activities are regulated by cell cycle and growth pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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