Thermo Fisher Scientific DTYMK Monoclonal Antibody (OTI2A1), TrueMAB
DTYMK 단백질을 인식하는 마우스 단클론 항체로, Western blot과 ICC/IF에 사용 가능. 정제된 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. DNA 합성 관련 효소 연구 및 항암제 기전 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A1 |
| Immunogen | Full length human recombinant protein of human DTYMK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.
DTYMK is essential for DNA synthesis and is a key enzyme in the pathway of several pyrimidine analog drugs, including 5-Fluorouracil (5-FU), a chemotherapeutic agent used for various solid tumors.
The human DTYMK gene was identified through functional complementation of a Saccharomyces cerevisiae cell cycle mutant (cdc8).
DTYMK mRNA levels and enzyme activities are regulated by cell cycle and growth pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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