Thermo Fisher Scientific MEK1 C-Term Polyclonal Antibody, DyLight 800
DyLight 800 형광 표지된 MEK1 C-말단 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 사람, 생쥐, 랫트에서 반응하며 MEK2와는 반응하지 않습니다. 고순도 친화 크로마토그래피 정제 제품으로 안정적인 형광 신호를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| Conjugate | DyLight™ 800 |
| Excitation / Emission Max | 789 / 794 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This DyLight™ 800 conjugated affinity purified antibody is directed against human MEK1 protein. Cross-reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen.
This antibody does not react with the MEK2 isoform.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation.
MAP2K1 is activated by various growth factors, cytokines, and by membrane-dependent depolarization and calcium influx.
Mek1 is activated by phosphorylation of serine 218 and 222 residues by Raf1.
It participates in signaling during stress response, apoptosis, and proliferative induction by cytokines.
Mutations in the gene can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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