Thermo Fisher Scientific GST Polyclonal Antibody, DyLight 405
DyLight 405 형광 표지된 GST 폴리클로날 항체로 Western blot, ICC/IF, ELISA에 적합. Schistosoma japonicum 유래 GST를 면역원으로 사용. 높은 특이성과 감도를 제공하며, 400/423 nm 파장에서 최적 형광 신호 발현. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length GST isolated from Schistosoma japonicum |
| Conjugate | DyLight™ 405 |
| Excitation / Emission Max | 400 / 423 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Product is stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum).
Cross-reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein present in both eukaryotes and prokaryotes, where it catalyzes a variety of reactions.
Glutathione S-transferase is used to create the GST gene fusion system, allowing purification of GST-fusion proteins via their affinity for glutathione.
Fusion proteins are valuable tools for studying direct protein–protein interactions.
The GST gene fusion system has been introduced into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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