Thermo Fisher Scientific GST Polyclonal Antibody, DyLight 405

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:25,000

-

Immunocytochemistry (ICC/IF)

>1:5,000

-

ELISA (ELISA)

>1:20,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The immunogen is full length GST isolated from Schistosoma japonicum.

Conjugate

DyLight™ 405 DyLight™ 405 DyLight™ 405

Excitation/Emission Max

400/423 nm View spectra

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.