Thermo Fisher Scientific MYC Epitope Tag Polyclonal Antibody, DyLight 405
MYC 에피토프 태그를 인식하는 DyLight 405 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 감도로 태그 단백질 검출에 유용. 400/423 nm 형광 특성으로 다중 검출 실험에 활용 가능.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified from whole rabbit serum prepared by repeated immunizations with MYC epitope tag peptide (E-Q-K-L-I-S-E-E-D-L) conjugated to KLH using maleimide. Sequence corresponds to amino acids 410–419 of human c-Myc. |
| Conjugate | DyLight™ 405 |
| Excitation/Emission Max | 400/423 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only immediately before use.
This affinity-purified antibody is directed against the MYC epitope tag and is useful for detecting overexpressed proteins in various assays.
It recognizes the MYC epitope tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either amino- or carboxy-termini of target proteins in transfected or transformed cells.
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation.
It forms a heterodimer with MAX, binding to the E-box DNA consensus sequence to regulate transcription of specific target genes.
Amplification of this gene is frequently observed in human cancers.
Translocations involving MYC are associated with Burkitt lymphoma and multiple myeloma.
Translation initiates from both an upstream non-AUG (CUG) and a downstream AUG start site, producing two isoforms with distinct N-termini.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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