Thermo Fisher Scientific V5 Epitope Tag Polyclonal Antibody, DyLight 405

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:25,000

-

Immunocytochemistry (ICC/IF)

>1:5,000

-

ELISA (ELISA)

>1:20,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 95-108 (G-K-P-I-P-N-P-L-L-G-L-D-S-T) of the V protein conjugated to KLH using maleimide.

Conjugate

DyLight™ 405 DyLight™ 405 DyLight™ 405

Excitation/Emission Max

400/423 nm View spectra

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against the V5 epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the V5 epitope tag (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.