Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), Brilliant Ultra Violet 737

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test
Publications: -

Product Specifications

Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j (ILT2, LILRB1, LIR1), a member of the ILT/LIR/MIR family.
CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs that signal through SHP-1 interactions.
Expression is found on myeloid cells (monocytes, dendritic cells) and some lymphoid cells (NK, T, B cells).
It is correlated with leukemias (ALL, CLL) and viral infections (HIV, EBV, CMV).
Ligands include MHC Class I molecules such as HLA-G, A, F, B27, E, and F.

HP-F1 reduces CD16-dependent cytolytic activity of NK cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry of normal human peripheral blood cells.
Use at 5 µL (1.0 µg) per test (final volume 100 µL). Cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm; use with UV (355 nm) laser.
Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401)
• Brilliant Stain Buffer (Product # 00-4409-75)

Light Sensitivity: Protect vial and samples from light.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Store fixed samples up to 3 days at 4°C in dark.
BUV737 is compatible with short-term methanol fixation (<1 hour).

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD85j belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at chromosome 19q13.4.
It encodes a transmembrane receptor with extracellular immunoglobulin domains and cytoplasmic ITIM motifs.
Expressed on immune cells, it binds MHC class I molecules and transduces inhibitory signals to regulate immune response, inflammation, and cytotoxicity.
Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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